Reference Gene Validation for Quantitative RT-PCR during Biotic and Abiotic Stresses in Vitis vinifera
نویسندگان
چکیده
منابع مشابه
Reference Gene Validation for Quantitative RT-PCR during Biotic and Abiotic Stresses in Vitis vinifera
Grapevine is one of the most cultivated fruit crop worldwide with Vitis vinifera being the species with the highest economical importance. Being highly susceptible to fungal pathogens and increasingly affected by environmental factors, it has become an important agricultural research area, where gene expression analysis plays a fundamental role. Quantitative reverse transcription polymerase cha...
متن کاملHousekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress.
Plant stress studies are more and more based on gene expression. The analysis of gene expression requires sensitive, precise, and reproducible measurements for specific mRNA sequences. Real-time RT-PCR is at present the most sensitive method for the detection of low abundance mRNA. To avoid bias, real-time RT-PCR is referred to one or several internal control genes, which should not fluctuate d...
متن کاملRecommended Reference Genes for Quantitative PCR Analysis in Soybean Have Variable Stabilities during Diverse Biotic Stresses.
For real-time reverse transcription-PCR (qRT-PCR) in soybean, reference genes in different tissues, developmental stages, various cultivars, and under stress conditions have been suggested but their usefulness for research on soybean under various biotic stresses occurring in North-Central U.S. is not known. Here, we investigated the expression stabilities of ten previously recommended referenc...
متن کاملEvaluation of reference genes for quantitative RT-PCR in Lolium temulentum under abiotic stress
Lolium temulentum L. (Darnel ryegrass) is a valuable model grass species for the study of stress in forage and turf grasses. Gene expression analysis by quantitative real-time RT-PCR relies on the use of proper internal standards. The aim of this studywas to identify and evaluate reference genes for use in real-time quantitative RT-PCR for abiotic stress studies in L. temulentum. Partial sequen...
متن کاملMolecular Characteristics and Biochemical Functions of VpPR10s from Vitis pseudoreticulata Associated with Biotic and Abiotic Stresses
Grapes are one of the world's oldest and most important fruit crops. They are of high economic value in many countries, but the susceptibility of the dominant winegrape species Vitis vinifera to fungal disease is a significant problem. The Chinese wild grape species are a rich source of disease-resistance genes and these can be used to discover how disease resistance in V. vinifera grapevines m...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLoS ONE
سال: 2014
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0111399